c2c12 cells Search Results


c2c12 cell line  (CLS Cell Lines Service GmbH)
92
CLS Cell Lines Service GmbH c2c12 cell line
C2c12 Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 myoblasts  (Genecopoeia)
95
Genecopoeia c2c12 myoblasts
C2c12 Myoblasts, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cells  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology c2c12 cells
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12  (Innovative Research Inc)
90
Innovative Research Inc c2c12
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse myoblastic c2c12 cells  (Dainippon Sumitomo)
90
Dainippon Sumitomo mouse myoblastic c2c12 cells
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
Mouse Myoblastic C2c12 Cells, supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cells  (BioResource International Inc)
90
BioResource International Inc c2c12 cells
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse c2c12 myoblasts  (KAC Co Ltd)
90
KAC Co Ltd mouse c2c12 myoblasts
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
Mouse C2c12 Myoblasts, supplied by KAC Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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skeletal muscle cell line c2c12 myotubes  (Procell Inc)
90
Procell Inc skeletal muscle cell line c2c12 myotubes
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
Skeletal Muscle Cell Line C2c12 Myotubes, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 myotubes  (Dawley Inc)
90
Dawley Inc c2c12 myotubes
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Myotubes, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 myogenic cell line  (LGC Promochem)
90
LGC Promochem c2c12 myogenic cell line
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Myogenic Cell Line, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cell line  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures c2c12 cell line
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c2c12 cell line  (Mediatech)
90
Mediatech c2c12 cell line
miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) <t>C2C12</t> cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction
C2c12 Cell Line, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) C2C12 cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction

Journal: Diabetologia

Article Title: MicroRNA-193b impairs muscle growth in mouse models of type 2 diabetes by targeting the PDK1/Akt signalling pathway

doi: 10.1007/s00125-021-05616-y

Figure Lengend Snippet: miR-193b decreases activation of Akt by inhibiting PDK1 expression. ( a ) Graphical representation of the conserved miR-193b binding motifs within the 3′-UTR of Pdk1 . Complementary sequences to the seed regions of miR-193b within the 3′-UTRs are conserved between human (Homo) and mouse (Mus) sequences. ( b , c ) C2C12 cells were treated with miR-193b mimic or control (Ctrl) RNA and qPCR analysis was used to determine the mRNA level of PDK1 ( n = 5). ** p < 0.01, *** p < 0.001 vs time 0 ( b ) or Ctrl RNA treatment alone ( c ), by one-way ANOVA with Bonferroni correction. ( d ) Luciferase (luc) activity of the reporter constructs containing either wild-type or mutated (MT) 3′-UTR of murine Pdk1 after treatment of C2C12 cells with miR-193b mimic or Ctrl RNA ( n = 5). *** p < 0.001 vs Ctrl RNA treatment alone in the 3′-UTR-transfected group, by one-way ANOVA with Bonferroni correction. ( e ) C2C12 cells were treated with miR-193b mimic (40 nmol/l) or miR-193b inhibitor (100 nmol/l) and the protein level of PDK1, Akt and p-Akt was detected by western blot analysis. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( f ) Pdk1 siRNA was transfected into C2C12 cells. Cells were then treated with miR-193b inhibitor or nontargeting negative Ctrl RNA and western blot analysis was used to determine the protein level of PDK1, Akt and p-Akt. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). ( g ) C2C12 cells were treated with DEX alone or with a combination of miR-193b inhibitor and DEX and the protein level of PDK1, Akt and p-Akt was detected by western blot. Quantification of the relative levels of PDK1 and p-Akt proteins is shown ( n = 3). In ( e – g ): * p < 0.05, ** p < 0.01, *** p < 0.001 vs untreated cells, by one-way ANOVA with Bonferroni correction

Article Snippet: C2C12 cells were transfected with 3′ untranslated region (UTR) luciferase reporter constructs ( Pdk1 3′-UTR or Pdk1 3′-UTR-mutant), miRNA (control RNA [catalogue no.: sc-36,869; Santa Cruz Biotechnology] or miR-193b-3p) and Renilla luciferase using Lipofectamine 3000 (Invitrogen).

Techniques: Activation Assay, Expressing, Binding Assay, Control, Luciferase, Activity Assay, Construct, Transfection, Western Blot