Journal: Cancer Cell International

Article Title: In vitro myotoxic effects of bupivacaine on rhabdomyosarcoma cells, immortalized and primary muscle cells

doi: 10.1186/s12935-015-0229-6

Figure Lengend Snippet: Microscopic images of a C2C12 cells; b primary muscle cells and c rhabdomyosarcoma cells.

Article Snippet: Rhabdomyosarcoma cells (CLS-Cell Lines Service, Eppelheim, Germany), immortalized C2C12 (CLS-Cell Lines Service, Eppelheim, Germany) muscle cells and primary muscle cells were used for the study.

Techniques:

Journal: Cancer Cell International

Article Title: In vitro myotoxic effects of bupivacaine on rhabdomyosarcoma cells, immortalized and primary muscle cells

doi: 10.1186/s12935-015-0229-6

Figure Lengend Snippet: Calculated bupivacaine concentration (ppm) with 50% cell survival (IC50)

Article Snippet: Rhabdomyosarcoma cells (CLS-Cell Lines Service, Eppelheim, Germany), immortalized C2C12 (CLS-Cell Lines Service, Eppelheim, Germany) muscle cells and primary muscle cells were used for the study.

Techniques: Concentration Assay

Journal: Cancer Cell International

Article Title: In vitro myotoxic effects of bupivacaine on rhabdomyosarcoma cells, immortalized and primary muscle cells

doi: 10.1186/s12935-015-0229-6

Figure Lengend Snippet: Combined calculated IC50 bupivacaine concentrations (ppm) differentiating only cell type

Article Snippet: Rhabdomyosarcoma cells (CLS-Cell Lines Service, Eppelheim, Germany), immortalized C2C12 (CLS-Cell Lines Service, Eppelheim, Germany) muscle cells and primary muscle cells were used for the study.

Techniques:

Journal: Toxics

Article Title: Selenium Nanoparticles Attenuate Cobalt Nanoparticle-Induced Skeletal Muscle Injury: A Study Based on Myoblasts and Zebrafish

doi: 10.3390/toxics12020130

Figure Lengend Snippet: Cellular toxicity induced by cobalt and selenium nanoparticles. ( A ) Cells were treated with 5 μg/mL FITC-labeled CoNPs and SeNPs for 2 h. Confocal microscopy revealed fluorescent signals into the cells. Yellow arrows indicating cells that are filled with green fluorescence, while the culture medium contains free FITC-labeled CoNPs and SeNPs. ( B – D ) Viability of C2C12 cells treated with 5–80 μg/mL CoNPs, SeNPs, and mixing of CoNPs and SeNPs were determined by CCK-8. Data are presented as mean ± standard deviation of three identical experiments conducted in triplicate. * Statistically significant difference compared with the controls ( p < 0.05 for each).

Article Snippet: According to the manufacturer’s instructions, the levels of MDA (malondialdehyde), GSH (glutathione), and SOD (superoxide dismutase) in C2C12 cells were measured using a GSH ELISA Kit (Elabscience, Wuhan, China), MDA Content Detection Kit (Beyotime, Nantong, China), and SOD Activity Detection Kit (Beyotime, Nantong, China).

Techniques: Labeling, Confocal Microscopy, Fluorescence, CCK-8 Assay, Standard Deviation

Journal: Toxics

Article Title: Selenium Nanoparticles Attenuate Cobalt Nanoparticle-Induced Skeletal Muscle Injury: A Study Based on Myoblasts and Zebrafish

doi: 10.3390/toxics12020130

Figure Lengend Snippet: Protective effects of low-dose SeNPs on CoNP-induced oxidative stress in muscle cells. ( A ) C2C12 cells were exposed to control, 20 μg/mL CoNPs, 500 µM H2O2, 5 μg/mL SeNPs with or without H2O2, and mixing of Co and Se NPs. Oxidative stress condition induced by H2O2 treatment for 4 h. Intracellular ROS production was analyzed by H2DCFDA staining. ( B – E ) MDA, GSH, and SOD levels were determined in C2C12 cells from control, 20 μg/mL CoNPs, 500 µM H2O2, 5 μg/mL SeNPs with or without H2O2, and mixing of Co and Se NP-treated groups. Data are displayed as mean ± SD; * means p < 0.05 between two indicated groups (n = 10).

Article Snippet: According to the manufacturer’s instructions, the levels of MDA (malondialdehyde), GSH (glutathione), and SOD (superoxide dismutase) in C2C12 cells were measured using a GSH ELISA Kit (Elabscience, Wuhan, China), MDA Content Detection Kit (Beyotime, Nantong, China), and SOD Activity Detection Kit (Beyotime, Nantong, China).

Techniques: Control, Staining

Journal: Toxics

Article Title: Selenium Nanoparticles Attenuate Cobalt Nanoparticle-Induced Skeletal Muscle Injury: A Study Based on Myoblasts and Zebrafish

doi: 10.3390/toxics12020130

Figure Lengend Snippet: CoNP-induced apoptosis was inhibited by SeNPs in muscle cells. ( A , B ) The expression of caspase–3 and cleaved caspase–3 in C2C12 cells treated with 5 μg/mL SeNPs or 20 μg/mL CoNPs with or without SeNPs was analyzed by Western blotting. GAPDH was used as internal reference. Relative expression levels are shown in the graph. ( C , D ) The degree of apoptosis of cells subjected to different treatments was determined by annexin V-FITC/PI double-staining flow cytometry. The percentage of apoptotic cells was counted and compared between the different groups. Data are displayed as mean ± SD; * means p < 0.05 between two indicated groups (n = 3).

Article Snippet: According to the manufacturer’s instructions, the levels of MDA (malondialdehyde), GSH (glutathione), and SOD (superoxide dismutase) in C2C12 cells were measured using a GSH ELISA Kit (Elabscience, Wuhan, China), MDA Content Detection Kit (Beyotime, Nantong, China), and SOD Activity Detection Kit (Beyotime, Nantong, China).

Techniques: Expressing, Western Blot, Double Staining, Flow Cytometry

Journal: Toxics

Article Title: Selenium Nanoparticles Attenuate Cobalt Nanoparticle-Induced Skeletal Muscle Injury: A Study Based on Myoblasts and Zebrafish

doi: 10.3390/toxics12020130

Figure Lengend Snippet: Promotive effects of SeNPs on CoNP-induced inhibition of myogenic differentiation. ( A ) Immunofluorescence staining in SeNPs, CoNPs, and Co Se NP-treated C2C12 cells was analyzed at 4 days of differentiation to detect the effect of different nanoparticles on myogenic differentiation. Nuclei (DAPI, blue), F-actin (Green), and α-SMA (Red) were labeled; scale bar = 25 µm. ( B ) Quantification of α-SMA fluorescence intensity in ( A ) was performed. Data are displayed as mean ± SD. * means p < 0.05 between two indicated groups (n = 6). ( C – E ) mRNA expression of myogenic markers, MyoD, myogenin, and Myf5, in C2C12 cells treated with 5 μg/mL SeNPs, 20 μg/mL CoNPs with or without SeNPs was analyzed by qPCR. The control group was set to 1.0. Data are displayed as mean ± SD. * means p < 0.05 between two indicated groups (n = 6).

Article Snippet: According to the manufacturer’s instructions, the levels of MDA (malondialdehyde), GSH (glutathione), and SOD (superoxide dismutase) in C2C12 cells were measured using a GSH ELISA Kit (Elabscience, Wuhan, China), MDA Content Detection Kit (Beyotime, Nantong, China), and SOD Activity Detection Kit (Beyotime, Nantong, China).

Techniques: Inhibition, Immunofluorescence, Staining, Labeling, Fluorescence, Expressing, Control

Journal: ACS Omega

Article Title: Phytochemical Screening and Antidiabetic Efficacy of Balanites aegyptiaca Seed Extract and Their Silver Nanoparticles on Muscle and Pancreatic Cell Lines

doi: 10.1021/acsomega.4c00327

Figure Lengend Snippet: Histogram showing the mean viability of C2C12 cells (a) and MIN6 cells (b) exposed with the different concentration of seed extracts and AgNPs seeds of B. aegyptiaca . Results as mean ± standard error; ** Significance level with control ( p ≤ 0.001), # Significance level with control ( p ≤ 0.001), and ## Significance level with control ( p ≤ 0.0001).

Article Snippet: The acquisition of C2C12 and MIN6 cells took place at the National Centre for Cell Sciences in Pune, Maharashtra, featuring passage numbers of 20 and 24, respectively.

Techniques: Concentration Assay, Control

Journal: ACS Omega

Article Title: Phytochemical Screening and Antidiabetic Efficacy of Balanites aegyptiaca Seed Extract and Their Silver Nanoparticles on Muscle and Pancreatic Cell Lines

doi: 10.1021/acsomega.4c00327

Figure Lengend Snippet: Histogram showing the percent glucose uptake in C2C12 cells (as of control), exposed with the different concentration of seeds extract, AgNPs seeds (a) and fractions (b) obtained from the seed extract of B. aegyptiaca Results are expressed as mean ± SE of three independent experiments performed with three replicates. ## Represents that the results are significantly different from the control with a significance level of ( p ≤ 0.0001).

Article Snippet: The acquisition of C2C12 and MIN6 cells took place at the National Centre for Cell Sciences in Pune, Maharashtra, featuring passage numbers of 20 and 24, respectively.

Techniques: Control, Concentration Assay